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KMID : 0368420150580050293
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Journal of Plant Biology
2015 Volume.58 No. 5 p.293 ~ p.302
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Knock-down of OsLOX by RNA interference leads to improved seed viability in rice
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Bai Suyang
He Niqing
Zhou Lu
Shen Beibei
Wu Wei
Liu Xi
Jiang Ling
Wan Jianmin
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Abstract
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Previous work found that lipoxygenases were key enzymes in lipid peroxidation, which causes grain deterioration during storage. In order to obtain better seed viability in rice, 10 marker-free knock-down lines were obtained in the progeny of endogenous OsLOX knock-down mutations caused by the RNAi technology. After artificial accelerated aging, there were four types of knock-down lines with higher seed viability than wild type (receptor parent). OsLOX3 knock-down line NPF1 was of special interest. In a series of experiments, including Southern blots, analysis of OsLOX3 expression, and enzymatic activity, NPF1 had better seed viability than wild-type. We also investigated the main agronomic characters of both knock-down lines and non-transgenetic wild type families. Knock-down lines were identified with generally excellent agronomic characteristics similar to the wild-type.
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KEYWORD
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Agronomic characters, Enzymatic activity, Gene expression, Knock-down lines, Lipoxygenase, Seed viavility
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